A Novel Enzyme-Linked Immunosorbent Assay for Quantification of Soybean .BETA.-Conglycinin, a Major Soybean Storage Protein, in Soybean and Soybean Food Products
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چکیده
منابع مشابه
beta-Conglycinin and glycinin in high-protein soybean seeds.
The agronomic performance and storage proteins of high seed protein lines of soybeans [Glycine max L. (Merr.)] were investigated to determine if the two major storage proteins, beta-conglycinin and glycinin, contribute to the increased protein content of high seed protein lines. Subunits of these two major storage proteins were estimated by scanning SDS-PAGE gels by scanning densitometry. The r...
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Four hundred fifty day old unsexed broiler chicks were used in a 35-day trial at Chittagong veterinary and animal sciences university farm to find out the effect of using crude soybean oil sediment (CSOS) as a substitute for refined soybean oil (RSO) in broiler diet. The experiment was carried out following completely randomized design. The chicks were randomly distributed in three treatment gr...
متن کاملNoteMeasurement of Gly m Bd 30 K , a Major Sandwich Enzyme - linked ImmunosorbentSoybeanAssayAllergen , in Soybean Products
derrnatitis, we found 16 allergenic proteins in the soybean. i) About two-thirds of soybean-sensitive patients examined was shown to have IgE antibodies binding to the 30-kDa protein narned Gly m Bd 30K. This finding suggests that Gly m Bd 30K is a major allergen in the soybean and that it may be a target allergen for hypoallergenization of soybean products. Gly m Bd 30K has been purified to ho...
متن کاملAll Three Subunits of Soybean -Conglycinin Are Potential Food Allergens
Soybeans are recognized as one of the “big 8” food allergens. IgE antibodies from soybean-sensitive patients recognize more than 15 soybean proteins. Among these proteins only the R-subunit of -conglycinin, but not the highly homologous R′and -subunits, has been shown to be a major allergenic protein. The objective of this study was to examine if the R′and -subunits of -conglycinin can also ser...
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ژورنال
عنوان ژورنال: Journal of Nutritional Science and Vitaminology
سال: 2005
ISSN: 0301-4800,1881-7742
DOI: 10.3177/jnsv.51.34